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hybrid-seq  (Epigenomics ag)

 
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    Epigenomics ag hybrid-seq
    Hybrid Seq, supplied by Epigenomics ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid-seq/product/Epigenomics ag
    Average 90 stars, based on 1 article reviews
    hybrid-seq - by Bioz Stars, 2026-06
    90/100 stars

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    Image Search Results


    (Left) Genome Assembly: High-accuracy ccs reads that were generated from PacBio subreads and trimmed Nanopore reads were de novo assembled to create draft genomes. Contigs were selected, and chromosome names were assigned based on the P. yoelii 17X reference genome alignment. Further processing of the Nanopore + Illumina hybrid assembly involved implementing scaffolding and iterative polishing. (Right) Gene-model prediction: A Nanopore dRNA-seq-based gene model and a hybrid gene model combining both Nanopore dRNA-seq and Illumina RNA-seq data were generated using Braker2. The predicted genes were annotated using reciprocal BLAST against P. yoelii 17X proteins. Illumina RNA-seq reads were previously reported .

    Journal: bioRxiv

    Article Title: Long-Read Genome Assembly and Gene Model Annotations for the Rodent Malaria Parasite Plasmodium yoelii 17XNL

    doi: 10.1101/2023.01.06.523040

    Figure Lengend Snippet: (Left) Genome Assembly: High-accuracy ccs reads that were generated from PacBio subreads and trimmed Nanopore reads were de novo assembled to create draft genomes. Contigs were selected, and chromosome names were assigned based on the P. yoelii 17X reference genome alignment. Further processing of the Nanopore + Illumina hybrid assembly involved implementing scaffolding and iterative polishing. (Right) Gene-model prediction: A Nanopore dRNA-seq-based gene model and a hybrid gene model combining both Nanopore dRNA-seq and Illumina RNA-seq data were generated using Braker2. The predicted genes were annotated using reciprocal BLAST against P. yoelii 17X proteins. Illumina RNA-seq reads were previously reported .

    Article Snippet: Even without any polishing efforts, this approach outperformed a parallel effort using a hybrid Nanopore long-read DNA-seq/Illumina short-read DNA-seq method by several key metrics, including its assembly quality and the reduction of gaps.

    Techniques: Generated, Scaffolding, RNA Sequencing

    Comparison of tissue versus ctDNA for detection of PIK3CA mutations according to technique and performance.

    Journal: Therapeutic Advances in Medical Oncology

    Article Title: The diagnostic accuracy of PIK3CA mutations by circulating tumor DNA in breast cancer: an individual patient data meta-analysis

    doi: 10.1177/17588359221110162

    Figure Lengend Snippet: Comparison of tissue versus ctDNA for detection of PIK3CA mutations according to technique and performance.

    Article Snippet: , Plasma , Hybrid capture-based NGS (Hi-Seq, Illumina) (FoundationACT ctDNA assay) , E542K; E545K; H1047R; H1047L , E545K (1); H1047L (1); H1047R (1); E726K (1) , ctDNA− , 0 , 11 , 11 , Specificity , 100.

    Techniques: Comparison, Mutagenesis, Diagnostic Assay, Clinical Proteomics, Sequencing, Digital PCR, Amplification, Hybridization